3P081 Changes in thermodynamic properties caused by amino acid substitutions in lattice proteins

Amino Acid Properties and Consequences of Substitutions

Detection of neutral amino acid substitutions in proteins.

The field of biochemical genetics relies heavily upon the detection by electrophoresis of genetically determined variants of proteins. Most of these variants differ by substitutions that involve charged amino acids. Genetic variants of another large class, ones that involve substitutions among neutral amino acids, are not easily detected and are often ignored. Ampholyte isoelectric focusing in ...

Increased thermodynamic stability by hydrophilic amino acid substitutions on the surface of Streptomyces subtilisin inhibitor

Streptomyces subtilisin inhibitor (SSI) is a homodimer composed of 113 amino acid residues in a subunit and contains three Met residues at the positions of 70, 73, and 103. Met73 is the P1 site residue on the surface of SSI and is crucial for inhibitory activity. We have studied the relationship between the thermal stability and hydrophobicity of the P1 site residue. Mutant proteins were prepar...

Parallel functional changes in the digestive RNases of ruminants and colobines by divergent amino acid substitutions.

A morphological or physiological trait may appear multiple times in evolution. At the molecular level, similar protein functions may emerge independently in different lineages. Whether these parallel functional changes are due to parallel amino acid substitutions has been a subject of debate. Here, I address this question using digestive ribonucleases (RNases) of two groups of foregut-fermentin...

Scoring Amino Acid Substitutions In Φhage Genomes

Substitution matrices are among the most widely used scoring techniques : BLAST, Muscle and other alignment packages, all use them. However these matrices are general; they ignore organism specific properties and do not provide customized scoring schemes. We present a Φhage-specific scoring matrix based on the abundances of aligned substitutions. These matrices use information from approximatel...